--- title: "From Synthesis to Final Product: Yield, Deprotection, and Purification Explained" description: "From Synthesis to Final Product: Yield, Deprotection, and Purification Explained" image: https://blog.sierrabio.com/hubfs/business%20graph%20of%20success%20in%20blues%20and%20black%20colours%20with%20nice%20light%20effects.jpeg --- [0 items in quote](https://sierrabio.com/request-a-quote/) No products in the Quote Basket. 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A 100-base oligo may produce less than 40% crude yield — before purification. ### Improving Coupling Efficiency - Eliminate moisture - Optimize reagent volumes and timing - Choose appropriate solid supports - Maintain fresh reagents ### Cleavage and Deprotection Cleavage releases the oligo from the solid support, while deprotection removes chemical protecting groups. Conditions vary based on oligo type and modifications. RNA oligos require an additional **2′-deprotection step**, making protocol selection especially important. ### Purification Options Common methods include: - Desalting (low purity) - Cartridge purification - Reverse-phase HPLC - Anion-exchange HPLC - PAGE for long or highly structured oligos The right method depends on oligo length, application, and purity requirements. ### Final Thought Understanding yield, purification, and analytical methods ensures you select the correct synthesis scale and avoid costly rework. ## Share this post [mailto:https%3A%2F%2Fblog.sierrabio.com%2Ffrom-synthesis-to-final-product-yield-deprotection-and-purification-explained](mailto:https%3A%2F%2Fblog.sierrabio.com%2Ffrom-synthesis-to-final-product-yield-deprotection-and-purification-explained) ## Keep reading ### [![](https://blog.sierrabio.com/hs-fs/hubfs/Chemist%20holding%20up%20beaker%20of%20green%20chemical%20in%20the%20laboratory.jpeg?width=1000&height=667&name=Chemist%20holding%20up%20beaker%20of%20green%20chemical%20in%20the%20laboratory.jpeg) Lab Setup, Safety, and Compliance for In-House Oligo Synthesis](https://blog.sierrabio.com/lab-setup-safety-and-compliance-for-in-house-oligo-synthesis) ### [![](https://blog.sierrabio.com/hs-fs/hubfs/Large-Scale-Apparatus.jpg?width=900&height=696&name=Large-Scale-Apparatus.jpg) Choosing the Right Oligonucleotide Synthesizer for Your Lab](https://blog.sierrabio.com/choosing-the-right-oligonucleotide-synthesizer-for-your-lab) [![](https://blog.sierrabio.com/hs-fs/hubfs/JPG%20file%20-02.jpg?width=2000&height=1125&name=JPG%20file%20-02.jpg)](http://sierrabio.com) --- Privacy Policy · Legal · © 2026 Sierra BioSystems, Inc.. All rights reserved. - [Home](https://sierrabio.com/) - [About Sierra BioSystems](https://sierrabio.com/about-sierra-biosystems/) - [Terms & Privacy Policy](https://sierrabio.com/privacy-policy/) - [My Account](https://sierrabio.com/my-account/) - [Contact](https://sierrabio.com/contact/) © 2026 Copyright Sierra BioSystems, Inc. ```json { "@context" : "https://schema.org", "@type" : "BlogPosting", "author" : { "@type" : "Person", "name" : "Harold Devoz", "url" : "https://blog.sierrabio.com/author/harold-devoz" }, "dateModified" : "2026-04-03T19:43:51.647Z", "datePublished" : "2026-03-03T16:00:02.000Z", "headline" : "From Synthesis to Final Product: Yield, Deprotection, and Purification Explained", "image" : [ "https://blog.sierrabio.com/hubfs/business%20graph%20of%20success%20in%20blues%20and%20black%20colours%20with%20nice%20light%20effects.jpeg" ], "mainEntityOfPage" : { "@id" : "https://blog.sierrabio.com/from-synthesis-to-final-product-yield-deprotection-and-purification-explained", "@type" : "WebPage" }, "publisher" : { "@type" : "Organization", "logo" : { "@type" : "ImageObject", "url" : "https://blog.sierrabio.com/hubfs/JPG%20file%20-02.jpg" }, "name" : "Sierra BioSystems, Inc." } } ```